Cell division and reproduction

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In order for cells to increase in number, either for growth or replacement of old cells, they go through the process of cell division – the cell cycle. Although the cycle is generally considered to consist of three major phases, there is a fourth phase to cell life.

G0 is the “fourth” state, where the cell is not waiting to divide but is just doing its cellular thing. It may stay in this state indefinitely or eventually enter into the first phase of the cell cycle.

The cell cycle.. PPMAT = Prophase, Prometaphase, Metaphase, Anaphase and Telophase. Thick black lines are checkpoints. Author’s own work.

The interphase

The first major phase of the cell cycle is the so-called interphase, in which the cell fulfills its normal activities while preparing for cell division. The interphase is itself divided into three steps.

  1. During G1, or the first gap, the cell procures and stores DNA, proteins and energy for the task ahead.
  2. During the S (synthesis) phase, DNA replication takes place. Chromatin is condensed into chromosomes, and the two copies – sister chromatids – are attached at a point called the centromere. The cell’s centrosome, the organizing center for microtubules, is duplicated.
  3. The G2 (gap 2) phase is another moment for storing energy and making proteins. Also, the cytoskeleton is dismantled and some organelles are replicated.

Mitosis

The mitosis phase does all the rest of the replication and localization of organelles, leaving only the actual division of the cell in two. It too is understood to take place in a number of steps

Prophase

Prophase

Prometaphase_1

Prometaphase

Metaphase

Metaphase

Anaphase

Anaphase

Telophase

Telophase

The phases of mitosis in animal cells, from Gray’s Anatomy1)Henry Gray, Anatomy of the Human Body (1918). via Wikimedia Commons

 

  1. During the first phase, prophase, the two centrosomes move towards opposite sides of the cell and microtubules form between them an array of links called the mitotic spindle. The nuclear envelope breaks up and various organelles move to the sides. The sister chromatids condense even more.
  2. During the prometaphase, condensation of chromosomes and establishment of the mitotic spindle continue. A protein complex called a kinetochore connects each sister chromatid to the microtubules of the spindle.
  3. During the metaphase, the still condensing chromosomes are lined up in the equatorial plane of the cell, the metaphase plate.
  4. The actual split of chromosomes takes place in the anaphase2)Why is it not called the cataphase?, with sister chromatids pulled towards opposite poles of the cell by the kinetochores, which crawl along the spindle tubules much as myosin crawls along actin during muscle contraction.
  5. During the telophase, nuclear envelopes form around the two separate chromosomes and the mitotic spindles break down in preparation for making new cytoskeletons.
Animal-cell mitosis and cytokinesis, from Openstax College

Animal-cell mitosis and cytokinesis, from Openstax College

Cytokinesis

The actual division of the cell into two takes place differently in plant and animal cells, since plant cells are surrounded by a stiff outer cell wall.

In animal cells, a ring of actin filaments contracts around the center of the cell and pinches it in two.

In plant cells, the Golgi apparatus has stored up enzymes, proteins and glucose molecules during the interphase. During the telophase, these components move along microtubules to the metaphase plate. There, they grow and fuse and eventually form a new cell wall.

Checkpoints

At several points in the cell cycle, there are checkpoints at which the readiness of the cell to enter the next part of the cycle is controlled. For instance, if the DNA is not intact, the whole process may either be restarted or canceled altogether. The cell may actually be instructed to commit apoptosis, cell suicide.

If ever, due to a mutation or bad gene copy, for instance, a checkpoint does not function properly, the defective DNA will be passed on to daughter cells. This may lead to the unchecked replication of such cells and genes – cancer.

Reproduction — meiosis

Most animals and plants are diploid, meaning they have two complete copies of all their chromosomes (genes), one from each parent. Although cell growth and doubling depends on the cell cycle and mitosis, reproduction of the organism depends on another cycle, meiosis.

Note that eukaryotes participate in both mitosis and meiosis:

  • Mitosis grows new cells identical to the original. A diploid (or 2n) cell duplicate itself into two diploid (2n) cells.

diploid cell → (mitosis) → two diploid cells

  • Meiosis, for reproduction, creates two haploid cells from one diplod cell.

diploid cell → (meiosis) → two haploid cells

Eukaryotic meiosis takes place in two cyclical parts, meiosis I and meiosis II. How it all works is sketched in the following figure,

At the top of the figure (a) are shown two homologous chromosomes, meaning that they have the same chromosome number (between 1 and 23) and so correspond to the same genes. For clarity, they are labeled 1 and 2. Suppose that one (colored blue) comes from the father and the other (pink), from the mother.

During the S part of interphase, duplicates of all chromosomes are made, called, as in mitosis. sister chromatids. The two blue chromosomes (1 and 3) are sisters and so are the two pink ones (2 and 4). All four are homologous. Sister chromatids are bound together at the centromere and will remain so bound until meiosis II.

During prophase I (prophase of meiosis I), the two pairs, which are now visible under a microscope, are closely and precisely aligned, a pairing called synapsis (c). Being homologous, a gene on a pink chromosome is aligned with the corresponding gene on a blue one. The proximity of the two allows crossing over, or recombination, to take place (d), the reciprocal exchange of genetically equivalent sequences of DNA between two non-sister chromosomes, in this case numbers 1 and 2. Genes are exchanged between the chromosomes of the father and those of the mother. Since this is random, it is the first source of randomness in the resulting chromosome. The synapsis then loosens (e), but the pairs remain linked at the chiasmata (singular = chiasma), the loci where crossing over has taken place. The four chromosomes are said to constitute a tetrad. In general, a sister chromatid is no longer composed of genes from only one parent, but a mix of maternal and paternal genes.

Meiosis in animals3)Author’s own work.

It can happen that a piece of one chromosome may break loose and join on to another. Such translocations have been observed in persons suffering from a specific kind of leukemia.

At the end of prophase I, homologous, non-sister chromatids are held together at chiasmata; sister chromatids, at centromeres.

During prometaphase I and metaphase I, microtubules of the spindle from the centrosomes, which are now on opposite side of the cell, attach via kinetochores to the centromeres of the chromosomes, i.e., to sister pairs. These connections are also random. Any two of the 23 chromosomes may be pulled to one side and the other two to the other side. This introduces a second source of randomness in the final product. In the case of human beings with 23 chromosomes, there are 223 – over eight million – possibilities.

Next, during anaphase I, the spindle fibers pull the chromosomes to either side, with sisters breaking apart at the chiasmata, but remaining together, since the kinetochores are attached to the centromeres.

In the cytokinesis phase, the two cells separate. In our case, chromosomes numbers 1 and 3 remain together, as well as 2 and 4. Each cell contains two chromosomes, but they are sisters, so the cell is considered to be haploid.

In summary, at the end of meiosis I, parental genes have been exchanged on chromosomes by crossing over, and the distribution of parental gens is mixed by their random distribution to the two resultant cells, rather like cards which are shuffled and then dealt into two decks.

During meiosis II, a kinetochore is formed on each sister chromatid. In anaphase II, the two chromatids are pulled apart and nuclear envelopes form around them. Cytokinesis finishes the separation, leaving only one chromosome in each cell, which is therefore haploid. Each one contains a random mix of maternal and paternal genes and may form a unique gamete (egg or sperm cell) of the species.

Mitosis assures growth and cell replacement. It serves reproduction in some simple organisms. Sexual reproduction, as in an animal, requires meiosis.

Now let’s go see how meiosis and mitosis work in plant life cycles and take a look at the science of taxonomy.

 

Notes   [ + ]

1. Henry Gray, Anatomy of the Human Body (1918)
2. Why is it not called the cataphase?
3. Author’s own work.